DOAJ Open Access 2022

Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics

Sarang S. Talwelkar Iris A.K. Lähdeniemi Mikko I. Mäyränpää Annabrita Hemmes Nora Linnavirta +4 lainnya

Abstrak

Summary: Drug sensitivity data acquired from solid tumor-derived cultures are often unsuitable for personalized treatment guidance due to the lengthy turnaround time. Here, we present a protocol for determining ex vivo drug sensitivities using fresh uncultured human lung tumor-derived EpCAM+ epithelial cells (FUTCs). We describe steps for drug testing in FUTCs to identify tumor cell-selective single or combination therapy in 72 h of sample processing. The FUTC-based approach can also be used to predict in vivo resistance to known targeted therapies.For complete details on the use and execution of this protocol, please refer to Talwelkar et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (9)

Sarang S. Talwelkar

Iris A.K. Lähdeniemi

Mikko I. Mäyränpää

Annabrita Hemmes

Nora Linnavirta

Jari Räsänen

Aija Knuuttila

Krister Wennerberg

Emmy W. Verschuren

Format Sitasi

APA MLA BibTeX

Talwelkar, S.S., Lähdeniemi, I.A., Mäyränpää, M.I., Hemmes, A., Linnavirta, N., Räsänen, J. et al. (2022). Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics. https://doi.org/10.1016/j.xpro.2022.101720

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Informasi Jurnal

Tahun Terbit: 2022
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2022.101720
Akses: Open Access ✓