DOAJ Open Access 2022

Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning

Bhaskar Jyoti Borah Chi-Kuang Sun

Abstrak

Summary: A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol, we provide a design guideline to enable high-NFOM MPM imaging while simultaneously securing a large FOV/digital-resolution ratio and a fast resonant raster-scanning speed. We further provide a free version of our custom acquisition software to assist with a smooth and easy construction process.For complete details on the use and execution of this protocol, please refer to Borah et al. (2021).

Topik & Kata Kunci

Science (General)

Penulis (2)

Bhaskar Jyoti Borah

Chi-Kuang Sun

Format Sitasi

APA MLA BibTeX

Borah, B.J., Sun, C. (2022). Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning. https://doi.org/10.1016/j.xpro.2022.101330

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Informasi Jurnal

Tahun Terbit: 2022
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2022.101330
Akses: Open Access ✓