DOAJ Open Access 2021

Employing nanobodies for immune landscape profiling by PET imaging in mice

Abraham J.P. Teunissen Omar B. Abousaway Jazz Munitz Mandy M.T. van Leent Yohana C. Toner +5 lainnya

Abstrak

Summary: Noninvasive immunoimaging holds great potential for studying and stratifying disease as well as therapeutic efficacy. Radiolabeled single-domain antibody fragments (i.e., nanobodies) are appealing probes for immune landscape profiling, as they display high stability, rapid targeting, and excellent specificity, while allowing extremely sensitive nuclear readouts. Here, we present a protocol for radiolabeling an anti-CD11b nanobody and studying its uptake in mice by a combination of positron emission tomography imaging, ex vivo gamma counting, and autoradiography. Our protocol is applicable to nanobodies against other antigens.For complete details on the use and execution of this protocol, please see Priem et al. (2020), Senders et al. (2019), or Rashidian et al. (2017).

Topik & Kata Kunci

Science (General)

Penulis (10)

Abraham J.P. Teunissen

Omar B. Abousaway

Jazz Munitz

Mandy M.T. van Leent

Yohana C. Toner

Bram Priem

Max L. Senders

Carlos Pérez-Medina

Willem J.M. Mulder

Mohammad Rashidian

Format Sitasi

APA MLA BibTeX

Teunissen, A.J., Abousaway, O.B., Munitz, J., Leent, M.M.v., Toner, Y.C., Priem, B. et al. (2021). Employing nanobodies for immune landscape profiling by PET imaging in mice. https://doi.org/10.1016/j.xpro.2021.100434

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Informasi Jurnal

Tahun Terbit: 2021
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2021.100434
Akses: Open Access ✓