DOAJ Open Access 2021

Live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices

Xiaoyi Qu Atul Kumar Francesca Bartolini

Abstrak

Summary: Analyses of microtubule (MT) plus end dynamics at glutamatergic en passant boutons can be carried out in cultured primary neurons isolated from mouse or rat embryos or ex vivo in acute slices isolated from mice that had been electroporated in utero. Here, we describe a protocol for setting up and analyzing live image recordings of primary neurons and acute hippocampal slices expressing tagged versions of the MT plus end binding protein EB3 and the presynaptic vesicle markers vGlut1 or VAMP2.For complete information on the use and execution of this protocol, please refer to Qu et al. (2019).

Topik & Kata Kunci

Science (General)

Penulis (3)

Xiaoyi Qu

Atul Kumar

Francesca Bartolini

Format Sitasi

APA MLA BibTeX

Qu, X., Kumar, A., Bartolini, F. (2021). Live imaging of microtubule dynamics at excitatory presynaptic boutons in primary hippocampal neurons and acute hippocampal slices. https://doi.org/10.1016/j.xpro.2021.100342

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Informasi Jurnal

Tahun Terbit: 2021
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2021.100342
Akses: Open Access ✓