DOAJ Open Access 2020

Detection of Activated Mouse Neurons with Temporal Resolution via Dual c-Fos Staining

Thiago Seike Nakahara Vinicius Miessler de Andrade Carvalho Mateus Augusto de Andrade Souza Guilherme Ziegler Trintinalia Fabio Papes

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Abstrak

Summary: This protocol combines fluorescent in situ hybridization and immunostaining to simultaneously detect, in histological sections from the same animal, subpopulations of neurons activated after two episodes of sensory stimulation. It allows the identification of groups of cells singly activated by either stimulus or co-activated by both stimuli. Our method results in nuclear staining for c-Fos mRNA and c-Fos protein, allowing better spatial and temporal resolution than previously published protocols, although it requires quick brain fixation.For complete details on the use and execution of this protocol, please refer to Carvalho et al. (2015, 2020).

Topik & Kata Kunci

Science (General)

Penulis (5)

Thiago Seike Nakahara

Vinicius Miessler de Andrade Carvalho

Mateus Augusto de Andrade Souza

Guilherme Ziegler Trintinalia

Fabio Papes

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Nakahara, T.S., Carvalho, V.M.d.A., Souza, M.A.d.A., Trintinalia, G.Z., Papes, F. (2020). Detection of Activated Mouse Neurons with Temporal Resolution via Dual c-Fos Staining. https://doi.org/10.1016/j.xpro.2020.100153

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Informasi Jurnal

Tahun Terbit: 2020
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2020.100153
Akses: Open Access ✓