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DOAJ Open Access 2022

In vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products

Jacqueline Miller-Holt Irene Baskerville-Abraham Masanori Sakimura Toshiro Fukushima Andrea Puglisi +1 lainnya
Lihat Sumber DOI

Abstrak

Non-clinical in vitro studies were conducted to investigate the characteristics of extracts from tobacco free nicotine pouches alongside a reference snus product and/or 1R6F reference cigarette. In vitro investigations were conducted in the Neutral Red Uptake (NRU) cytotoxicity assay, Bacterial Reverse Mutation (Ames) assay, and in vitro Mammalian Cell Micronucleus (ivMN) assay. These products were also investigated for their oral irritation potential in the EpiGingival™ 3D tissue model. Results from the Ames, in vitro Micronucleus and NRU assays indicated that the tested products were non-mutagenic, non-genotoxic and non-cytotoxic in contrast to results obtained for the 1R6F reference cigarette. Results from Complete Artificial Saliva (CAS) extracts from these products also failed to be classified as irritants (as measured using the MTT assay), in the EpiGingival™ 3D tissue model

Topik & Kata Kunci

Toxicology. Poisons

Penulis (6)

J

Jacqueline Miller-Holt

I

Irene Baskerville-Abraham

M

Masanori Sakimura

T

Toshiro Fukushima

A

Andrea Puglisi

J

Jeremie Gafner

Format Sitasi

APA MLA BibTeX
Miller-Holt, J., Baskerville-Abraham, I., Sakimura, M., Fukushima, T., Puglisi, A., Gafner, J. (2022). In vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products. https://doi.org/10.1016/j.toxrep.2022.06.008

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Informasi Jurnal
Tahun Terbit
2022
Sumber Database
DOAJ
DOI
10.1016/j.toxrep.2022.06.008
Akses
Open Access ✓