DOAJ Open Access 2024

Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation

Hongmei Xin Yixiao Li Kaihui Zhang Aihua Ji Meili Fan

Abstrak

A human induced pluripotent stem cell (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC. The iPSC line expresses pluripotency markers, displays a normal karyotype, and is able to differentiate into three germ layers in vitro. This iPSC line represents a valuable cell model for NF1 in humans.

Topik & Kata Kunci

Biology (General)

Penulis (5)

Hongmei Xin

Yixiao Li

Kaihui Zhang

Aihua Ji

Meili Fan

Format Sitasi

APA MLA BibTeX

Xin, H., Li, Y., Zhang, K., Ji, A., Fan, M. (2024). Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation. https://doi.org/10.1016/j.scr.2024.103568

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Informasi Jurnal

Tahun Terbit: 2024
Sumber Database: DOAJ
DOI: 10.1016/j.scr.2024.103568
Akses: Open Access ✓