DOAJ Open Access 2024

Induced pluripotent stem cell (iPSC) line MLi005-A derived from a patient with dominant dystrophic epidermolysis bullosa (DDEB)

Sandra Ropret Preeti Khurana Tara Fabčič Janina Cvetkovska Ana Trobec +5 lainnya

Abstrak

We have generated MLi005-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a male patient with dominant dystrophic epidermolysis bullosa (DDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi005-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-005A line has been also tested for ability to differentiate into fibroblasts and keratinocytes and markers associated with these cell types.

Topik & Kata Kunci

Biology (General)

Penulis (10)

Sandra Ropret

Preeti Khurana

Tara Fabčič

Janina Cvetkovska

Ana Trobec

Špela Zemljič Jokhadar

Dusko Ilic

John A McGrath

Christina Guttmann-Gruber

Mirjana Liovic

Format Sitasi

APA MLA BibTeX

Ropret, S., Khurana, P., Fabčič, T., Cvetkovska, J., Trobec, A., Jokhadar, Š.Z. et al. (2024). Induced pluripotent stem cell (iPSC) line MLi005-A derived from a patient with dominant dystrophic epidermolysis bullosa (DDEB). https://doi.org/10.1016/j.scr.2024.103306

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Informasi Jurnal

Tahun Terbit: 2024
Sumber Database: DOAJ
DOI: 10.1016/j.scr.2024.103306
Akses: Open Access ✓