DOAJ Open Access 2022

Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system

Thi-Hong Nhung Nguyen Thi-Tuyet Trinh Tran Thi-Hoa Luong Xuan-Hung Nguyen

Abstrak

We have established the footprint-free Vietnamese human induced pluripotent stem cell (hiPSC) line, VRISGi002-A, from CD71 + CD235a + erythroid progenitor cells (EPCs) of a 27-year-old healthy donor. The EPCs were enriched from isolated peripheral blood and reprogrammed using Sendai viruses which carried the reprogramming factors c-MYC, SOX2, KLF4, and OCT4 under a feeder-free culture system. The established VRISGi002-A cell line expressed typical pluripotency markers, displayed a normal karyotype, and demonstrated the potential to differentiate into the three germ layers. This hiPSC line could serve as a Vietnamese healthy control model for physiological processes and drug screening.

Topik & Kata Kunci

Biology (General)

Penulis (4)

Thi-Hong Nhung Nguyen

Thi-Tuyet Trinh Tran

Thi-Hoa Luong

Xuan-Hung Nguyen

Format Sitasi

APA MLA BibTeX

Nguyen, T.N., Tran, T.T., Luong, T., Nguyen, X. (2022). Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system. https://doi.org/10.1016/j.scr.2022.102824

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Informasi Jurnal

Tahun Terbit: 2022
Sumber Database: DOAJ
DOI: 10.1016/j.scr.2022.102824
Akses: Open Access ✓