DOAJ Open Access 2017

Development of one control and one tumor-specific induced pluripotent stem cell line from laryngeal carcinoma patient

Yamin Zhang Jing Zhu Yaozhang Dai Liuzhong Wang Rui Liu

Abstrak

Skin fibroblasts and tumor fibroblasts were extracted from a 64-year old male patient clinically diagnosed with laryngeal carcinoma. Control and tumor specific induced pluripotent stem cells were reprogrammed with 5 reprogramming factors, Klf-4, c-Myc, Oct-4, Sox-2, and Lin-28, using the messenger RNA reprogramming system. The transgene-free iPSC lines showed pluripotency, confirmed by immunofluorescence staining. The iPSC lines also showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. This in vitro cellular model can be used to study the oncogenesis and pathogenesis of laryngeal carcinoma.

Topik & Kata Kunci

Biology (General)

Penulis (5)

Yamin Zhang

Jing Zhu

Yaozhang Dai

Liuzhong Wang

Rui Liu

Format Sitasi

APA MLA BibTeX

Zhang, Y., Zhu, J., Dai, Y., Wang, L., Liu, R. (2017). Development of one control and one tumor-specific induced pluripotent stem cell line from laryngeal carcinoma patient. https://doi.org/10.1016/j.scr.2017.07.027

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Informasi Jurnal

Tahun Terbit: 2017
Sumber Database: DOAJ
DOI: 10.1016/j.scr.2017.07.027
Akses: Open Access ✓