Green synthesis, characterization, and in vitro biomedical applications of Diospyros blancoi synthesized copper and zinc oxide nanoparticles

This research describes a green synthesis method for creating nanoparticles that uses copper (Cu) and zinc (Zn) ions in combination with leaf extract from Diospyros blancoi. It creates an ecologically benign alternative to traditional synthesis methods by using the bioactive phytochemicals found in Diospyros blancoi leaves, which function as natural reducing and stabilizing agents. UV–Vis spectroscopy, FTIR, TEM, and XRD determined the produced nanoparticles' structural, optical, and antibacterial characteristics. The creation of nanoparticles in the presence of Cu and zinc doping, which alters their size and shape, is confirmed by the distinctive absorption peaks at 250 nm on the UV–Vis spectra. Functional groups coming from plants were identified by FTIR analysis as aiding in the production and stability of nanoparticles. The morphological variations between the nanoparticles made with copper oxide (CuO) and zinc oxide (ZnO) were highlighted by TEM pictures, which indicated the deposition of evenly dispersed nanoparticles ranging in size from 50 to 300 nm. In contrast to Zn-doped, amorphous samples, Cu-doped samples showed a high degree of crystallinity as seen by their XRD patterns. Significant antibacterial activity was demonstrated by experimental research against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli; CuO nanoparticles were more effective, especially against S. aureus. According to the results, synthesizing functionalized nanoparticles using Diospyros blancoi extracts in green synthesis may be an effective method used in water-saturated circumstances and the biomedical area.