Based On Covalent Bonds Bimetallic Organic Frameworks In Periodontitis Treatment

Aim or purpose: Periodontitis is a chronic inflammatory disease caused by dental plaque biofilm. This work developes a novel nanocomposite Ti/Mn-MOF-PA by integrating piezoelectric bimetal-organic framework (MOF) nanosheets with phenylboric acid (PBA) and the nitric oxide donor arginine (Arg). The aim is to investigate its antibacterial effects and immune modulation, addressing challenges in deep periodontitis treatment. Materials and methods: Ti/Mn-MOF NSs were characterized by TEM, XRD, XPS and PFM. Sonodynamic activity, NO release, and stability were assessed by UV-Vis spectrophotometry. A computational analysis based on first-principles calculations was conducted. Adhesion of Ti/Mn-MOF-PA to P. gingivalis was analyzed by TEM, flow cytometry, and confocal microscopy. Antibacterial effect were determined by CFU counting, SEM, and live/dead staining. Immunomodulatory effects were studied by IF staining and q-PCR. In vivo inflammatory regulation was verified by imaging and histological staining. Microbial communities were identified by 16S rDNA sequencing. Results: 1.Ti/Mn-MOF-PA have excellent acoustic properties, controllable nitric oxide release, and strong piezoelectric performance.2. They could modulate immunity by inhibiting NLRP3 inflammasome assembly and suppressing NF-κB activation, kill bacteria, and disrupt biofilms. 3.In vivo studies show reduced inflammation by lowering inflammatory factors and collagen degradation. Conclusions: Ti/Mn-MOF-PA nanocomposites exhibit strong acoustic dynamics, controlled NO release, and piezoelectric properties, which help kill bacteria, disrupt biofilms, reduce the release of inflammatory factors.By targeting subgingival pathogens and restoring microbiota balance they show robust antibacterial and anti-inflammatory effects in vivo.