Sensitive and rapid detection of bisphenol A using signal amplification nanoparticles loaded with anti-bisphenol A monoclonal antibody
Abstrak
Bisphenol A has been reported to be a ubiquitous contaminant, and exposure to this compound can lead to adverse effects in human health. In the study, monoclonal antibody against BPA (anti-BPA mAb) with high affinity (3.74 × 109 L/mol) secreted by cell line 2E3 was successfully screened. Inspired by the signal amplification of nanoparticles, anti-BPA mAbs were labeled with nano-materials including colloidal gold (AuNP) and gold nanoflowers (AuNF) for preparation of immunoprobes and AuNP-/AuNF-based test strips. The developed AuNP- and AuNF-based test strips achieved the rapid and sensitive detection of BPA within 10 min, with the limit of detection (LOD) of 25 μg/mL and 3.125 μg/mL, respectively. The detection result in BPA spiked samples measured by the proposed methods was consistent with that detected by LC-MS method. The preparation process of as-prepared test strip is time-saving and considered as ideal candidates method for rapid screening BPA in real samples.
Topik & Kata Kunci
Penulis (9)
Sumei Ling
Aidi Xu
Menghan Sun
Xiaoli Li
Yongming Huang
Yang Xu
Jianli Huang
Tingting Xie
Shihua Wang
Akses Cepat
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- 2024
- Sumber Database
- DOAJ
- DOI
- 10.1016/j.fochx.2024.101903
- Akses
- Open Access ✓