DOAJ Open Access 2016

Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli

Lisa Gerner Steffi Munack Koen Temmerman Ann-Marie Lawrence-Dörner Hüseyin Besir +5 lainnya

Abstrak

Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 ‘apo’, CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required and the proteins subsequently used to develop a fluorescence-based assay for drug binding to the kinase, “Using the fluorescent properties of STO-609 as a tool to assist structure-function analyses of recombinant CaMKK2” [1]. Keywords: CaMKK2, Calmodulin, Fermentation

Topik & Kata Kunci

Computer applications to medicine. Medical informatics Science (General)

Penulis (10)

Lisa Gerner

Steffi Munack

Koen Temmerman

Ann-Marie Lawrence-Dörner

Hüseyin Besir

Matthias Wilmanns

Jan Kristian Jensen

Bernd Thiede

Ian G. Mills

Jens Preben Morth

Format Sitasi

APA MLA BibTeX

Gerner, L., Munack, S., Temmerman, K., Lawrence-Dörner, A., Besir, H., Wilmanns, M. et al. (2016). Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli. https://doi.org/10.1016/j.dib.2016.06.033

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Informasi Jurnal

Tahun Terbit: 2016
Sumber Database: DOAJ
DOI: 10.1016/j.dib.2016.06.033
Akses: Open Access ✓