DOAJ Open Access 2023

Single-cell RNA sequencing identifies a population of human liver-type ILC1s

Benjamin Krämer Ansel P. Nalin Feiyang Ma Sarah Eickhoff Philipp Lutz +40 lainnya

Abstrak

Summary: Group 1 innate lymphoid cells (ILCs) comprise a heterogeneous family of cytotoxic natural killer (NK) cells and ILC1s. We identify a population of “liver-type” ILC1s with transcriptional, phenotypic, and functional features distinct from those of conventional and liver-resident NK cells as well as from other previously described human ILC1 subsets. LT-ILC1s are CD49a+CD94+CD200R1+, express the transcription factor T-BET, and do not express the activating receptor NKp80 or the transcription factor EOMES. Similar to NK cells, liver-type ILC1s produce IFN-γ, TNF-α, and GM-CSF; however, liver-type ILC1s also produce IL-2 and lack perforin and granzyme-B. Liver-type ILC1s are expanded in cirrhotic liver tissues, and they can be produced from blood-derived ILC precursors in vitro in the presence of TGF-β1 and liver sinusoidal endothelial cells. Cells with similar signature and function can also be found in tonsil and intestinal tissues. Collectively, our study identifies and classifies a population of human cross-tissue ILC1s.

Topik & Kata Kunci

Biology (General)

Penulis (45)

Benjamin Krämer

Ansel P. Nalin

Feiyang Ma

Sarah Eickhoff

Philipp Lutz

Sonia Leonardelli

Felix Goeser

Claudia Finnemann

Gudrun Hack

Jan Raabe

Michael ToVinh

Sarah Ahmad

Christoph Hoffmeister

Kim M. Kaiser

Steffen Manekeller

Vittorio Branchi

Tobias Bald

Michael Hölzel

Robert Hüneburg

Hans Dieter Nischalke

Alexander Semaan

Bettina Langhans

Dominik J. Kaczmarek

Brooke Benner

Matthew R. Lordo

Jesse Kowalski

Adam Gerhardt

Jörg Timm

Marieta Toma

Raphael Mohr

Andreas Türler

Arthur Charpentier

Tobias van Bremen

Georg Feldmann

Arne Sattler

Katja Kotsch

Ali T. Abdallah

Christian P. Strassburg

Ulrich Spengler

William E. Carson, III

Bethany L. Mundy-Bosse

Matteo Pellegrini

Timothy E. O’Sullivan

Aharon G. Freud

Jacob Nattermann

Format Sitasi

APA MLA BibTeX

Krämer, B., Nalin, A.P., Ma, F., Eickhoff, S., Lutz, P., Leonardelli, S. et al. (2023). Single-cell RNA sequencing identifies a population of human liver-type ILC1s. https://doi.org/10.1016/j.celrep.2022.111937

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Informasi Jurnal

Tahun Terbit: 2023
Sumber Database: DOAJ
DOI: 10.1016/j.celrep.2022.111937
Akses: Open Access ✓