DOAJ Open Access 2023

Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase II

Xiong Liu Yifang Wang Ziyi Weng Qinyi Xu Cefan Zhou +2 lainnya

Abstrak

Transient receptor potential (TRP) polycystin-3 (TRPP3) is a non-selective cation channel activated by Ca2+ and protons and is involved in regulating ciliary Ca2+ concentration, hedgehog signaling and sour tasting. The TRPP3 channel function and regulation are still not well understood. Here we investigated regulation of TRPP3 by calmodulin (CaM) by means of electrophysiology and Xenopus oocytes as an expression model. We found that TRPP3 channel function is enhanced by calmidazolium, a CaM antagonist, and inhibited by CaM through binding of the CaM N-lobe to a TRPP3 C-terminal domain not overlapped with the EF-hand. We further revealed that the TRPP3/CaM interaction promotes phosphorylation of TRPP3 at threonine 591 by Ca2+/CaM-dependent protein kinase II, which mediates the inhibition of TRPP3 by CaM.

Topik & Kata Kunci

Biology (General) Medicine (General)

Penulis (7)

Xiong Liu

Yifang Wang

Ziyi Weng

Qinyi Xu

Cefan Zhou

JingFeng Tang

Xing-Zhen Chen

Format Sitasi

APA MLA BibTeX

Liu, X., Wang, Y., Weng, Z., Xu, Q., Zhou, C., Tang, J. et al. (2023). Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase II. https://doi.org/10.1016/j.cellin.2023.100088

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Informasi Jurnal

Tahun Terbit: 2023
Sumber Database: DOAJ
DOI: 10.1016/j.cellin.2023.100088
Akses: Open Access ✓