Non-electric bioelectrical analog strategy by a biophysical-driven nano-micro spatial anisotropic scaffold for regulating stem cell niche and tissue regeneration in a neuronal therapy
Abstrak
The slow regenerating rate and misdirected axonal growth are primary concerns that disturb the curative outcome of peripheral nerve repair. Biophysical intervention through nerve scaffolds can provide efficient, tunable and sustainable guidance for nerve regrowth. Herein, we fabricate the reduced graphene oxide (rGO)/polycaprolactone (PCL) scaffold characterized with anisotropic microfibers and oriented nanogrooves by electrospinning technique. Adipose-derived stem cells (ADSCs) are seeded on the scaffolds in vitro and the viability, neural differentiation efficiency and neurotrophic potential are investigated. RGO/PCL conduits reprogram the phenotype of seeded cells and efficiently repair 15 mm sciatic nerve defect in rats. In summary, biophysical cues on nerve scaffolds are key determinants to stem cell phenotype, and ADSC-seeded rGO/PCL oriented scaffolds are promising, controllable and sustainable approaches to enable peripheral nerve regeneration.
Topik & Kata Kunci
Penulis (12)
Xiangyun Yao
Lei Zhan
Zhiwen Yan
Juehong Li
Lingchi Kong
Xu Wang
Huimin Xiao
Huiquan Jiang
Chen Huang
Yuanming Ouyang
Yun Qian
Cunyi Fan
Format Sitasi
Akses Cepat
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Cek di sumber asli →- Tahun Terbit
- 2023
- Sumber Database
- DOAJ
- DOI
- 10.1016/j.bioactmat.2022.05.034
- Akses
- Open Access ✓