DOAJ Open Access 2012

Amperometric Uric Acid Biosensor with Improved Analytical Performances Based on Alkaline-Stable H2O2 Transducer

Damir Iveković Matea Japec Matea Solar Nataša Živković

Abstrak

Electrodes modified with Prussian blue containing structurally incorporated Ni2+ ions were employed as the alkaline-stable H2O2 transducers in amperometric uric acid biosensors based on urate oxidase. The employed H2O2 transducers enabled operation of the biosensor at low cathodic potentials and in mild alkaline media, near the optimal pH of urate oxidase. At the pH value of 9.0 and working potential of -0.05 V, the biosensor exhibited a linear response towards uric acid in the concentration range of 2.5 to 200 μM, with the sensitivity of 26.9± 0.4 mAM-1 cm-2 and detection limit of 0.65 μM. The biosensor was successfully applied for determination of uric acid in samples of commercial dairy products.

Topik & Kata Kunci

Industrial electrochemistry Physical and theoretical chemistry

Penulis (4)

Damir Iveković

Matea Japec

Matea Solar

Nataša Živković

Format Sitasi

APA MLA BibTeX

Iveković, D., Japec, M., Solar, M., Živković, N. (2012). Amperometric Uric Acid Biosensor with Improved Analytical Performances Based on Alkaline-Stable H2O2 Transducer. https://doi.org/10.1016/S1452-3981(23)13951-4

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Informasi Jurnal

Tahun Terbit: 2012
Sumber Database: DOAJ
DOI: 10.1016/S1452-3981(23)13951-4
Akses: Open Access ✓