Development of a low-cost, disposable biosensor for sensitive quantification of C1 inhibitor in commercial serum

Abstract Hereditary angioedema (HAE), an autosomal dominant disease that may be fatal in the larynx and gastrointestinal tract, can affect the skin and mucosal surfaces. Indium tin oxide-polyethyleneterephthalate (ITO-PET) electrode based on biosensor is put up in this study to detect C1-Inhibitor (C1-INH). The ITO-PET electrodes were surface have undergone a cleaning procedure. Hydroxylation (NH4OH, H2O2, H2O) was applied to the electrode surfaces. The ITO-PET electrode surfaces were then treated with 3-Aminopropyltrimethoxysilane (3-APTES). Crosslinking with glutaraldehyde was the following step. The concentration of 3-APTES, the concentration of anti-C1-INH, and the length of time that C1-INH was incubated were the ideal conditions. It conducted immobilization, optimization, and analysis stlod udies using techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The linear range, repeatability, reproducibility, regeneration studies, single frequency studies (SFI), and storage of life of the biosensor were carried out in characterization studies. It was discovered that the linear range of the immunosensor was 2 fg/mL to 1500 fg/mL. It discovered the biosensor's (11 weeks) storage life. The LOD value calculated as a result of the study is 0.23 fg/mL and the LOQ value is 0.26 fg/mL. The biosensor was tested using the (SWV) square wave voltammetry technique (0–1.5 V, equal time: 2, frequency: 25 Hz, pulse size: 25 mV). The interaction between antibody C1-INH and C1-INH antigens was observed using a single frequency technique (SFI).The final stage was testing the biosensor on commercial serum studies.