DOAJ Open Access 2012

Effect of N6-benzyladenine and indole-3-butyric acid on photosynthetic apparatus of Orthosiphon stamineus plants grown in vitro

D. Stoyanova-Koleva M. Stefanova M. Zhiponova V. Kapchina-Toteva

Abstrak

The leaf structure and chloroplast ultrastructure of kidney tea (Orthosiphon stamineus Benth.) was studied in in vitro culture on standard MS medium supplemented with or without plant growth regulators (PGRs). The cytokinin N6-benzyladenine (BA) negatively affected the structure of the palisade parenchyma and chloroplast ultrastructure and increased the stomatal frequency of the adaxial epidermis. The auxin indole-3-butyric acid (IBA) did not modify the morphology of regenerated leaf tissues as well as the chloroplast ultrastructure. The effect of both PGRs applied in combination was manifested in well-differentiated mesophyll parenchyma, typical chloroplast ultrastructure and increased stomatal frequency on both leaf surfaces. This protocol can be suggested for further ex vitro propagation.

Topik & Kata Kunci

Biology (General) Plant ecology

Penulis (4)

D. Stoyanova-Koleva

M. Stefanova

M. Zhiponova

V. Kapchina-Toteva

Format Sitasi

APA MLA BibTeX

Stoyanova-Koleva, D., Stefanova, M., Zhiponova, M., Kapchina-Toteva, V. (2012). Effect of N6-benzyladenine and indole-3-butyric acid on photosynthetic apparatus of Orthosiphon stamineus plants grown in vitro. https://doi.org/10.1007/s10535-012-0060-z

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Informasi Jurnal

Tahun Terbit: 2012
Sumber Database: DOAJ
DOI: 10.1007/s10535-012-0060-z
Akses: Open Access ✓