Mitochondrial ATP concentration decreases immediately after glucose administration to glucose‐deprived hepatocytes
Abstrak
Hepatocytes can switch their metabolic processes in response to nutrient availability. However, the dynamics of metabolites (such as lactate, pyruvate, and ATP) in hepatocytes during the metabolic switch remain unknown. In this study, we visualized metabolite dynamics in primary cultured hepatocytes during recovery from glucose‐deprivation. We observed a decrease in the mitochondrial ATP concentration when glucose was administered to hepatocytes under glucose‐deprivation conditions. In contrast, there was slight change in the cytoplasmic ATP concentration. A decrease in mitochondrial ATP concentration was associated with increased protein synthesis rather than glycogen synthesis, activation of urea cycle, and production of reactive oxygen species. These results suggest that mitochondrial ATP is important in switching metabolic processes in the hepatocytes.
Topik & Kata Kunci
Penulis (8)
Saki Tsuno
Kazuki Harada
Mina Horikoshi
Marie Mita
Tetsuya Kitaguchi
Masami Yokota Hirai
Mitsuharu Matsumoto
Takashi Tsuboi
Akses Cepat
- Tahun Terbit
- 2024
- Sumber Database
- DOAJ
- DOI
- 10.1002/2211-5463.13744
- Akses
- Open Access ✓