CrossRef Open Access 2023 2 sitasi

Photodynamic Effects with 5-Aminolevulinic Acid on Cytokines and Exosomes in Human Peripheral Blood Mononuclear Cells from Patients with Crohn’s Disease

Kristian Espeland Andrius Kleinauskas Petras Juzenas Sagar Darvekar Vlada Vasovic +4 lainnya

Abstrak

Photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) which is the precursor of the photosensitizer protoporphyrin IX (PpIX) is an available treatment for several diseases. ALA-PDT induces the apoptosis and necrosis of target lesions. We have recently reported the effects of ALA-PDT on cytokines and exosomes of human healthy peripheral blood mononuclear cells (PBMCs). This study has investigated the ALA-PDT-mediated effects on PBMC subsets from patients with active Crohn’s disease (CD). No effects on lymphocyte survival after ALA-PDT were observed, although the survival of CD3−/CD19+ B-cells seemed slightly reduced in some samples. Interestingly, ALA-PDT clearly killed monocytes. The subcellular levels of cytokines and exosomes associated with inflammation were widely downregulated, which is consistent with our previous findings in PBMCs from healthy human subjects. These results suggest that ALA-PDT may be a potential treatment candidate for CD and other immune-mediated diseases.

Penulis (9)

Kristian Espeland

Andrius Kleinauskas

Petras Juzenas

Sagar Darvekar

Vlada Vasovic

Trond Warloe

Eidi Christensen

Jørgen Jahnsen

Qian Peng

Format Sitasi

APA MLA BibTeX

Espeland, K., Kleinauskas, A., Juzenas, P., Darvekar, S., Vasovic, V., Warloe, T. et al. (2023). Photodynamic Effects with 5-Aminolevulinic Acid on Cytokines and Exosomes in Human Peripheral Blood Mononuclear Cells from Patients with Crohn’s Disease. https://doi.org/10.3390/ijms24054554

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Informasi Jurnal

Tahun Terbit: 2023
Bahasa: en
Total Sitasi: 2×
Sumber Database: CrossRef
DOI: 10.3390/ijms24054554
Akses: Open Access ✓