CrossRef
Open Access
2018
QA-Bio PNGase F Non-denatured Glycoprotein Protocol v1
Mike Gibson
Abstrak
PNGase F cleaves N-linked (asparagine-linked) oligosaccharides from glycoproteins. The enzyme deaminates asparagine to aspartic acid, leaving the oligosaccharides intact. Denaturation increases the rate of cleavage. Most native proteins can still be completely N-deglycosylated but incubation time must be increased. The enzyme will remain fully active under reaction conditions (37˚C) for at least 96 hours. PNGase F will not remove oligosaccharides containing Alpha-(1,3)-linked core fucose commonly found on plant glycoproteins; for this purpose, use peptide N-glycosidase A.
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Mike Gibson
Akses Cepat
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Cek di sumber asli →Informasi Jurnal
- Tahun Terbit
- 2018
- Bahasa
- en
- Sumber Database
- CrossRef
- DOI
- 10.17504/protocols.io.seaebae
- Akses
- Open Access ✓