OT-2 PCR sample preparation protocol v2

This protocol is meant to create a PCR mastermix with 1 or more primer sets, distribute it in 1 or more 96-well plates, inoculate from 1 or more 96-well plates containing the template and run the reaction in an OT-2 thermocycler (if available). This protocol is run by using a LAP format script and its corresponding .xlsx file were different customizable variables such as volumes of transfer, type of plates, number of primers per set, and availability of OT-2 thermocycler are indicated. Specifically, this protocol provides a set of instructions or description of the LAP repository entry LAP-PCR-OT2-2.0.0. You can find the script and complementary information for this specific version of the protocol in the LAP entry link and GitHub link to LAP entry documents In our laboratory, this protocol has been used as part of the "High-throughput workflow for the genotypic characterization of transposon insertion library variants" also available in protocols.io to prepare arbitrary and spurious PCR samples. The current version incorporates the following modifications: Description of robot and protocol setup in a separate protocols.io entry (Setting and Customizing OT-2 for LAP Entries) When transferring the polymerase and primers, change tip every time it aspirates from source tubes Wells with controls can be anywhere in the plate New variable in Sheet 'ModuleVariables' called 'Max Volume Per Mix Tube In Shaker'