OT-2 PCR sample preparation protocol v1
Abstrak
This protocol is meant to perform samples preparation of PCR plates for several primer sets to the same samples, i.e, we will have for all the selected samples (we can set the initial well and number of samples) of all source plates the master mix with different primer sets (combination of primers, water and polymerase mix) in the final plates. The instructions for running this protocol are a python script for an Opentrons 2 robot and need a csv file with several variables setted making the protocol modular to reactives, volumes of transfer, type of plates, number of primers per set, etc. In our laboratory, this protocol has been used as part of the "High-throughput workflow for the genotypic characterization of transposon library variants" also available in protocols.io to prepare PCR samples
Penulis (4)
Ana Mariya Anhel
Lorea Alejaldre
Manuel Gimenez
Ángel Goñi-Moreno
Akses Cepat
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Cek di sumber asli →- Tahun Terbit
- 2022
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- en
- Sumber Database
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- DOI
- 10.17504/protocols.io.n92ldpyznl5b/v1
- Akses
- Open Access ✓