OT-2 Counter-Selection v2
Abstrak
This protocol is meant to select the samples from one or more source plate(s) that has 2 different selecting conditions: one condition gives higher values and the other lower values. From these selected samples this protocol create final plates with the samples alone or mixed with different reactive(s). The instructions for running this protocol are a python script for an Opentrons 2 robot and a excel file with several variables making the protocol modular to reactives, volumes of transfer, type of plates, etc. In our lab we are selecting samples by their after night OD in 2 different antibiotics and create PCR plates (with water) and 2 stock plates (with glycerol), but it can be used with other selection characteristics such as expression of GFP and with other types of reactives. This protocol description corresponds to the entry LAP-ColonyCounterSelection-OT2-1.0.0 of the LAP repository
Penulis (3)
Ana Mariya Anhel
Lorea Alejaldre
Ángel Goñi-Moreno
Akses Cepat
PDF tidak tersedia langsung
Cek di sumber asli →- Tahun Terbit
- 2023
- Bahasa
- en
- Sumber Database
- CrossRef
- DOI
- 10.17504/protocols.io.5qpvor5xdv4o/v2
- Akses
- Open Access ✓