OT-2 Counter-Selection v1
Abstrak
This protocol is meant to select the samples from 1 source plate that has been growing in different conditions and has different values for these different conditions, one condition gives higher values and the other lower values. From this selected samples this protocol create final plates with 2 types of reactives The instructions for running this protocol are a python script for an Opentrons 2 robot and need a csv file with several variables setted making the protocol modular to reactives, volumes of transfer, type of plates, etc. In our lab we are selecting samples by their after night OD in 2 different antibiotics and create PCR plates (with water) and stock plates (with glycerol), but it can be used with other selection characteristics as expression of GFP and with other types of reactives.
Penulis (1)
Ana Mariya Anhel
Akses Cepat
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Cek di sumber asli →- Tahun Terbit
- 2022
- Bahasa
- en
- Sumber Database
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- DOI
- 10.17504/protocols.io.5qpvor5xdv4o/v1
- Akses
- Open Access ✓