CrossRef
Open Access
2022
Western blot (NuPAGE and MES buffer) v1
Laura Smith
Abstrak
Cells were lysed in 1% Triton X 100 lysis buffer with protease and phosphatase inhibitors. Cell lysates were electrophoresed with NuPage™ Bis-Tris protein gels. Proteins were transferred to a PVDF membrane, blocked in 10% milk and treated with primary and secondary antibodies in 5% milk. Antibody binding was detected using the GE Healthcare Amersham™ electro-chemi-luminescence (ECL)™ Prime Western Blotting Detection Reagent.
Penulis (1)
L
Laura Smith
Akses Cepat
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Cek di sumber asli →Informasi Jurnal
- Tahun Terbit
- 2022
- Bahasa
- en
- Sumber Database
- CrossRef
- DOI
- 10.17504/protocols.io.261genwyyg47/v1
- Akses
- Open Access ✓