Label-free intratissue activity imaging of alveolar organoids with dynamic optical coherence tomography
Abstrak
An organoid is a three-dimensional (3D) in vitro cell culture emulating human organs. We applied 3D dynamic optical coherence tomography (DOCT) to visualize the intratissue and intracellular activities of human induced pluripotent stem cells (hiPSCs)-derived alveolar organoids in normal and fibrosis models. 3D DOCT data were acquired with an 840-nm spectral domain optical coherence tomography with axial and lateral resolutions of 3.8 μm (in tissue) and 4.9 μm, respectively. The DOCT images were obtained by the logarithmic-intensity-variance (LIV) algorithm, which is sensitive to the signal fluctuation magnitude. The LIV images revealed cystic structures surrounded by high-LIV borders and mesh-like structures with low LIV. The former may be alveoli with a highly dynamics epithelium, while the latter may be fibroblasts. The LIV images also demonstrated the abnormal repair of the alveolar epithelium.
Topik & Kata Kunci
Penulis (11)
Rion Morishita
Pradipta Mukherjee
Ibrahim Abd El-Sadek
Toshio Suzuki
Yiheng Lim
Antonia Lichtenegger
Shuichi Makita
Kiriko Tomita
Yuki Yamamoto
Tetsuharu Nagamoto
Yoshiaki Yasuno
Akses Cepat
- Tahun Terbit
- 2023
- Bahasa
- en
- Sumber Database
- arXiv
- Akses
- Open Access ✓