Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy
Abstrak
Cytochrome C oxidase and FoF1-ATP synthase constitute complex IV and V, respectively, of the five membrane-bound enzymes in mitochondria comprising the respiratory chain. These enzymes are located in the inner mitochondrial membrane (IMM), which exhibits large invaginations called cristae. According to recent cryo-tomography, FoF1-ATP synthases are located predominantly at the rim of the cristae, while cytochrome C oxidases are likely distributed in planar membrane areas of the cristae. Previous FLIM measurements (K. Busch and coworkers) of complex II and III unravelled differences in the local environment of the membrane enzymes in the cristae. Here, we tagged complex IV and V with mNeonGreen and investigated their mitochondrial nano-environment by FLIM and superresolution microscopy in living human cells. Different lifetimes and anisotropy values were found and will be discussed.
Topik & Kata Kunci
Penulis (9)
Franziska Foertsch
Mykhailo Ilchenko
Thomas Heitkamp
Silke Nossmann
Birgit Hoffmann
Ilka Starke
Ralf Mrowka
Christoph Biskup
Michael Borsch
Akses Cepat
- Tahun Terbit
- 2017
- Bahasa
- en
- Sumber Database
- arXiv
- Akses
- Open Access ✓