arXiv
Open Access
2016
Characterization of protein complexes using chemical cross-linking coupled electrospray mass spectrometry
Timothy D. Cummins
Gopal P. Sapkota
Abstrak
Identification and characterization of large protein complexes is a mainstay of biochemical toolboxes. Utilization of cross-linking chemicals can facilitate the capture and identification of transient or weak interactions of a transient nature. Here we describe a detailed methodology for cell culture based proteomic approach. We describe the generation of cells stably expressing green fluorescent protein (GFP)- tagged proteins under the tetracycline-inducible promoter and subsequent proteomic analysis of GFP-interacting proteins. We include a list of proteins that were identified as interactors of GFP.
Penulis (2)
T
Timothy D. Cummins
G
Gopal P. Sapkota
Akses Cepat
Informasi Jurnal
- Tahun Terbit
- 2016
- Bahasa
- en
- Sumber Database
- arXiv
- Akses
- Open Access ✓