Fluctuation analysis of mechanochemical coupling depending on the type of bio-molecular motor
Abstrak
Mechanochemical coupling was studied for two different types of myosin motors in cells: myosin V, which carries cargo over long distances by as a single molecule; and myosin II, which generates a contracting force in cooperation with other myosin II molecules. Both mean and variance of myosin V velocity at various [ATP] obeyed Michaelis-Menten mechanics, consistent with tight mechanochemical coupling. Myosin II, working in an ensemble, however, was explained by a loose coupling mechanism, generating variable step sizes depending on the ATP concentration and realizing a much larger step (200 nm) per ATP hydrolysis than myosin V through its cooperative nature at zero load. These different mechanics are ideal for the respective myosin's physiological functions.
Topik & Kata Kunci
Penulis (4)
Masatoshi Nishikawa
Hiroaki Takagi
Atsuko H. Iwane
Toshio Yanagida
Akses Cepat
- Tahun Terbit
- 2008
- Bahasa
- en
- Sumber Database
- arXiv
- Akses
- Open Access ✓